Genetics and Selection of Microorganisms

Major: Biotechnology and Bioengineering
Code of subject: 8.162.00.M.20
Credits: 3.00
Department: Technology of Biologically Active Substances, Pharmacy and Biotechnology
Lecturer: Associate Professor Komarovska-Porokhnyavets Olena Zoryanivna
Semester: 4 семестр
Mode of study: денна
Learning outcomes: • Ability to demonstrate in-depth knowledge of the basics of molecular biotechnology and bioengineering. • Search, analyze and critically evaluate information from various sources. • Assess the feasibility and feasibility of new methods and technologies in the development of biotechnological bases and methods of production of new biological products and biological products, improvement of existing ones.
Required prior and related subjects: prerequisites: • Academic entrepreneurship; • Planning and organization of research and statistical processing of results co-requisites: • Immuno- and DNA diagnostics and identification of GMOs; • Quality assessment of biological products and organic products
Summary of the subject: Features of genome organization prokaryotic organisms. Genetic apparatus of eukaryotic microorganisms. The structure of the genome of the yeast. Autonomous genetic elements in prokaryotes. Migrating genetic elements. IS-elements. Transposons. Conjugatic transposons. The mechanisms of replication, repairation and expression of the genome. Regulation of replication, transcription and translation in prokaryotes. Variability in prokaryotes. Mutagenesis. The essence of transformation, conjugation and transduction. The genetics of viruses. The structure and function of viral genomes. Replication, transcription and translation of viral genomes. Variability in viral genomes. Bacteriophages. Principles of construcnion of industrial microorganisms using methods of cellular and genetic engineering.
Assessment methods and criteria: • current control: oral examination, individual written tests (40%) • summary control: examination (exam) written-oral form (60%)
Recommended books: 1. Пиневич А. В. Микробиология: биология прокариотов: Т. 3. - СПб: Изд-во С.-Петерб. ун¬та, 2009. - 457 с 2. SARS coronavirus 2: from genome to infectome / M. Rastogi, N. Pandey, A. Shukla, Sunit K. Singh // Respiratory Research.- V. 21. – 2020. 3. Probable Pangolin Origin of SARS-CoV-2 Associated with the COVID-19 Outbreak / T. Zhang, Q.Wu, Z. Zhang // Current Biology. - V. 30, Issue 7. – 2020. - P. 1346 -1351. 4. Genome-wide analysis of SARS-CoV-2 virus strains circulating worldwide implicates heterogeneity / M. Rafiul Islam, M. Nazmul Hoque, M. Shaminur Rahman, A. S. M. Rubayet Ul Alam, Masuda Akther, J. Akter Puspo, Salma Akter, Munawar Sultana, Keith A. Crandall, M. Anwar Hossain // Scientific Reports. – 2020