Project of production technology of sodium salt of sulfopropyl ester of 4-acetylaminobenzenethiosulfonic acid
Students Name: Kozak Veronika Marianivna
Qualification Level: magister
Speciality: Pharmacy, Industrial Pharmacy
Institute: Institute of Chemistry and Chemical Technologies
Mode of Study: full
Academic Year: 2024-2025 н.р.
Language of Defence: ukrainian
Abstract: Kozak V.M., Vasilyuk S.V. (supervisor). “Project of technology for the production of sodium salt of sulfopropyl ester of 4-acetylaminobenzene thiosulfonic acid”. Master’s thesis. Lviv Polytechnic National University, Lviv, 2024. Organosulfur compounds have attracted considerable attention in antibacterial research due to their ability to disrupt bacterial cell walls without harming human cells [1]. Allicin, in particular, has demonstrated strong in vitro antibacterial activity against E. coli, S. aureus, S. pyrogenes, P. mirabilis, P. aeruginosa, A. Baumanii, K. pneumonie, and other saprophytic and pathogenic bacteria [2]. Sulfopropyl esters of thiosulfonic acids stand out for their unique chemical properties, making them promising compounds for a variety of industries and medicine. Due to the presence of functional groups that can enter into numerous reactions and interactions, these compounds can be used to create new materials, improve the effectiveness of pharmaceuticals, cosmetics, and fertilizers. This versatility makes them important components of the modern chemical and biotechnology industry. They also have great potential in the development of innovative medicines, which is particularly relevant in light of the growing resistance of pathogens to traditional antibiotics. Garlic is traditionally used to treat colds and various infectious diseases such as measles, chickenpox, and influenza [3]. Studies have shown that garlic extracts have antiviral activity in vitro against herpes simplex virus type 1 and influenza B virus [4]. Some organosulfur compounds demonstrate antifungal properties. Substances isolated from garlic have been found to have potent antifungal properties against Candida spp. and Aspergillus spp. In addition, pure allicin has been shown to be effective in inhibiting the growth of Microsporum, Cryptococcus, Trichophyton, Candida and Epidermophyton, as well as a good antioxidant [5]. The SuperPred online service is an effective tool for the initial stage of analyzing the biological activity of molecules. It allows narrowing down the range of potential targets and indications for further research and experiments, providing a hypothetical basis for the development of new drugs. Object of study: sodium salt of sulfopropyl ester of 4-acetylaminobenzene thiosulfonic acid. Research area: pharmacy, industrial pharmacy. Objective: to develop a project for the production of sodium salt of sulfopropyl ester of 4-acetylaminobenzene thiosulfonic acid. Screening of the biological activity of the sodium salt of sulfopropyl ester of 4-acetylaminobenzene sulfonic acid using SuperPred showed that the main target for this substance is the Pregnane X receptor, which is associated with the treatment of atherosclerosis, with a probability of 97.43% and model accuracy of 95%. It was also found to be highly likely to be associated with various biological targets related to cancer (e.g., breast, lung, prostate cancer), inflammatory processes, and metabolic disorders [6]. Taking into account the above, our task was to develop a project for the production of functionalized thiosulfonate, a sodium salt of sulfopropyl ester of 4-acetylaminobenzene thiosulfonic acid. Key words: sodium salt, predicted biological activity, synthesis, thiosulfonic acid esters. 1. Heldreth B, Turos E. (2005). Microbiological properties and modes of action of organosulfur-based anti-infectives. Curr Med Chem Anti-Infect Agents. 4:295–315 2. Goncharov N, Orekhov AN, Voitenko N et al. (2016.) Organosulfur compounds as nutraceuticals. In: Nutraceuticals. Elsevier, Oxford, pp 555–568 3. Khodavandi A, Alizadeh F, Aala F et al. (2010). In vitro investigation of anti-fungal activity of allicin alone and in combination with azoles against Candida species. Mycopathologia. https://doi. org/10.1007/s11046-009-9251-3 4.Lubenets V., Vasylyuk S., Monka N., Bolibrukh Kh., Komarovska-Porokhnyavets O., Baranovych D., Musyanovych R., Zaczynska E., Czarny A., Nawrot U., Novikov V. (2017). Synthesis and antimicrobial properties of 4-acylaminobenzenethiosulfoacid S-esters. Saudi Pharmaceutical Journal, 25(2), 266–274 DOI: 10.1016/j.jsps.2016.06.00 5. Liubas N., Iskra R., Stadnytska N., Monka N., Havryliak V., Lubenets V. (2022). Antioxidant Activity of Thiosulfonate Compounds in Experiments in Vitro and in vivo. Biointerface Research in Applied Chemistry, 12 (3), 3106 – 3116 https://doi.org/10.33263/BRIAC123.31063116 6. Kozak VM, Mykytyuk SR, Vasilyuk SV (2024). Prospects for the use of sodium salt of sulfopropyl ester of 4-acetylaminobenzene sulfonic acid for the treatment of diabetic complications. Appropriate solutions for gaps in pharmacy: in accordance with European priorities: collection of scientific papers of the International Student Scientific and Practical Conference. Lviv. November 14-15, 2024. - Lviv: Lviv Polytechnic Publishing House.