Проєкт технології виробництва аспарагінату заліза
Автор: Кулик Анастасія Василівна
Кваліфікаційний рівень: магістр
Спеціальність: Фармація, промислова фармація
Інститут: Інститут хімії та хімічних технологій
Форма навчання: заочна
Навчальний рік: 2023-2024 н.р.
Мова захисту: українська
Анотація: Karpukhina K.S.; Kurka M.S. (supervisor). Project of production technology of new antioxidants based on thiosemicarbazone derivatives. Master`s thesis. – Lviv Polytechnic National University, Lviv, 2023. This master’s thesis is devoted to the research of methods of synthesis of 5-(1-naphthyl)-furan-2-carbaldehyde thiosemicarbazone and the development of an industrial method for the production of a new antioxidant drug. 5-(1-Naphthyl)-furan-2-carbaldehyde of thiosemicarbazone was recognized as a leader compound among the synthesized derivatives of thiosemicarbazone X-furan-2-carbaldehyde obtained by the method described in the literature [1], therefore this derivative is proposed for introduction in industrial production. The synthesized thiosemicarbazone derivatives demonstrated a wide range of activities, including: in vitro antibacterial and antifungal activity against S. aureus and C. albicans was studied, while in vitro cytotoxic activity of these thiosemicarbazones was studied against several human tumor cell lines, antioxidant activity was studied using the method DPPH. In vitro studies have shown that 5-(1-naphthyl)-furan-2-carbaldehyde of thiosemicarbazone exhibits excellent antiproliferative activity with an IC50 value of 7.69 ?M, 5-fluorouracil is taken as a comparison drug, against seven tumor cell lines: H460 (large cell human lung carcinoma), HuTu80 (human duodenal adenocarcinoma), DU145 (human prostate carcinoma), MCF-7 (human breast adenocarcinoma), M-14 (human amelanotic melanoma), HT-29 (human colon adenocarcinoma), and also in LNCaP (human prostate adenocarcinoma) and in the non-neoplastic BALB/3T3 cell line. The obtained results indicate that cytotoxicity increases when groups of nitro- and naphthoyl substituents are attached to the furan ring at the C-5 position, respectively [2,3]. The analysis of literary sources made it possible to determine the technologically optimal scheme for the synthesis of 5-(1-naphthyl)-furan-2-carbaldehyde thiosemicarbazone, which is based on the condensation reaction of 5-naphthylfuran-2-carbaldehyde with thiosemicarbazide in a molar ratio of 1:1 in an ethanol solution. Thiosemicarbazone 5-(1-naphthyl)-furan-2-carbaldehyde was obtained as a solid, it was filtered, washed with ethanol, recrystallized from hot acetone and dried to remove moisture [1]. The main raw materials are characterized in the work, as well as a block diagram of production is drawn up. In the calculation part, the material calculations for the production of 5-(1-naphthyl)-furan-2-carbaldehyde thiosemicarbazone were carried out, as a result of which, taking into account the yield coefficients, the consumption rates of raw materials and semi-products were calculated for the production stages and as a whole for obtaining 1 ton of the finished product. On the basis of material calculations for a given production capacity, technological calculations were made and a basic technological scheme of production was developed [4,5]. In the work, the main device is selected, its characteristics are described, thermal calculations are carried out, and its drawings are given. The construction design of the planned production was carried out in accordance with the requirements and standards of Good Manufacturing Practice (GMP) and building norms and rules, which is presented in the form of drawings of the shop plan and its longitudinal and cross sections. In the economic calculations in this work, all material costs for obtaining 100 tons of finished product per year are taken into account, including: the wage fund, the cost of the main and auxiliary equipment, and the cost of raw materials, and the economic efficiency of the implementation of the proposed production is also shown. Research objects: 5-(1-naphthyl)-furan-2-carbaldehyde thiosemicarbazone. Field of research: pharmacy, industrial pharmacy, medicinal chemistry. The purpose of the research: study of methods of organic synthesis and properties of 5-(1-naphthyl)-furan-2-carbaldehyde thiosemicarbazone, development of technology for its industrial production, as well as study of ways of its use in medicine and pharmacy. Research results: a basic equipment-technological scheme for the production of 5-(1-naphthyl)-furan-2-carbaldehyde thiosemicarbazone was developed and the possibility of its introduction into production was shown, the main and auxiliary equipment was calculated and selected and its construction layout was proposed in accordance with the requirements and rules of GMP. and the economic efficiency of the developed production was also calculated. The work is laid out on 99 pages (without literary sources and appendices) and consists of an introduction, 4 chapters, conclusions and a list of used literature, contains references to more than 100 literary sources, 4 sheets of drawings are given in the appendices. Key words: thiosemicarbazide, 5-naphthylfuran-2-carbaldehyde, 5-(1-naphthyl)-furan-2-carbaldehyde thiosemicarbazone, industrial production technology, design. List of References: 1. Hernandez, W., Carrasco, F., Vaisberg, A., Spodine, E., Manzur, J., Icker, M., ... & Beyer, L. (2020). Synthesis, spectroscopic characterization, structural studies, and in vitro antitumor activities of pyridine-3-carbaldehyde thiosemicarbazone derivatives. Journal of Chemistry, 2020, 1-12. https://doi.org/10.1155/2020/2960165 2. Hernandez, W., Paz, J., Carrasco, F., Vaisberg, A., Spodine, E., Manzur, J., ... & Beyer, L. (2013). Synthesis and characterization of new palladium (II) thiosemicarbazone complexes and their cytotoxic activity against various human tumor cell lines. Bioinorganic Chemistry and Applications, 2013. https://doi.org/10.1155/2013/524701 3. Guler, S., Kayali, H. A., Sadan, E. O., Sen, B., & Subasi, E. (2022). Half-sandwich arene ruthenium (II) thiosemicarbazone complexes: evaluation of anticancer effect on primary and metastatic ovarian cancer cell lines. Frontiers in Pharmacology, 13, 882756. https://doi.org/10.3389/fphar.2022.882756 4. Protsesy i aparaty khimiko-farmatsevtychnoyi promyslovosti. Navch posibnyk dlya farm i khim spets. VNZ: Rekomendovano MON - Sydorov Yu.I., Chuyeshov V.I., Novikov V.P. -Vinnytsya: Nova Knyha, 2009. -816 s. 5. Tekhnolohichne obladnannya biotekhnolohichnoyi i farmatsevtychnoyi promyslovosti: pidruchnyk [dlya vyshch. navch. zakl.] Stasevych M.V., Mylyanych A.O., Strel?nykov L.S., Krut·s?kykh T.V., Buchkevych I.R., Zaytsev O.I., Huz?ova I.O., Strilets? O.P., Hladukh Ye.V., Novikov V.P. – L?viv: «Novyy Svit-2000», 2018. – 410 s.