Project of solizym production technology
Students Name: Malesh Yuliia Valentynivna
Qualification Level: magister
Speciality: Pharmaceutical Biotechnology
Institute: Institute of Chemistry and Chemical Technologies
Mode of Study: full
Academic Year: 2021-2022 н.р.
Language of Defence: ukrainian
Abstract: Malesh. Yu. V., Zvarych V. I. (supervisor). Project of solizym production technology. Master’s tesis.– Lviv Politechnic National University, Lviv, 2021 Extended abstract The object of research - the dried technical preparation of lipase. The field of research is the technology of separation of the drug from the culture fluid. The purpose of research is biosynthesis, the process of deep cultivation of the producer’s culture on a nutrient medium and the isolation of the final product. Microbiological enzyme preparations are increasingly replacing chemical catalysts in a number of industrial processes. Such drugs, in addition to environmental friendliness and high activity, have a number of advantages over enzyme preparations of plant and animal origin, namely: production of microbiological enzymes can be predicted and controlled; extracted microbiological enzymes are more stable in comparison with intracellular animal and plant enzymes; genetic flexibility of microorganisms allows to produce enzyme preparations with a wide range of specificity; microbiological enzymes can be synthesized over a long period of time, in contrast to seasonal plant enzymes. Enzymes are a product of fine microbiological synthesis that are widely used in industry, medicine and research. Since cheap substrates from other industries are used as substrates, it is realistic to obtain a high-value product based on cheap raw materials. This paper proposes a plan for the creation of production to obtain a technical enzyme preparation, with the possibility of modification for production to obtain purified enzymes of purity G10x and G20x. Lipase is a hydrolytic enzyme that is released from many organisms, such as animals, plants, fungi and bacteria. It breaks down triglycerides into monoglycerides and fatty acids, while having a wide substrate specificity. Lipases are used in the food industry and other areas of human activity. A characteristic feature of many lipases is the phenomenon of surface activation, which determines their inherent dependence of the rate of catalytic reaction on the concentration and physical state of the substrate. It is proved that the functioning of enzymes depends on their structural features. Internal cavities, tunnels and pores are integral components of the native conformation of the protein. They play an important role in the transport of substrate, cofactors and ions to the active and regulatory center of the enzyme. In addition, their configuration may affect the thermal stability of enzymes, and therefore the study of the above structures is necessary to understand the mechanisms of functioning of biocatalysts. Also, an important element of the structure of enzymes is the accumulation of charged and hydrophobic amino acid residues on their surface. This property must be taken into account when planning the ways of adsorption immobilization of biocatalysts on various media for use in industry and medicine. In the calculation part the material balance of all stages of production is executed. The technological scheme of the project with a high degree of control and the necessary level of automation was developed, which will ensure the output of a quality product. The calculation and layout of the main production facilities was carried out while maintaining the sequence of processes to prevent the intersection of flows and taking into account the peculiarities of the operation of some equipment. In the economic part of the work, technical and economic indicators were calculated and the economic feasibility of the project was proved. The conclusions formulated in the paper are in accordance with the literature. Key words: lipase, enzymes, product separation technology, project calculation.