Experimental Methods for Biomolecules Study

Major: Biotechnology and Bioengineering
Code of subject: 8.162.00.O.007
Credits: 4.00
Department: Technology of Biologically Active Substances, Pharmacy and Biotechnology
Lecturer: Professor, Ph.D., S.Sc. Havryliak Viktoriia
Semester: 2 семестр
Mode of study: денна
Learning outcomes: • Knowledge of modern research methods in the field of molecular biotechnology and bioengineering to create new biotechnological objects. • In-depth knowledge of modern physicochemical and biophysical methods for determining bioorganic drugs. • Argue the choice of methods for solving scientific and applied problems, critically evaluate the results and defend the decisions made.
Required prior and related subjects: prerequisites: Bioinformatics and information technologies in biotechnology co-requisites: Molecular biotechnology and bioengineering Plant biotechnology and bioengineering
Summary of the subject: The concept and definition of physico-chemical methods of analysis of bioorganic compounds. Polarimetry. Refractometry. Photometric methods. Types of spectrophotometry. Chromatography basics. Electrophoresis. Immune analysis. Nuclear magnetic resonance, its types. Mass spectrometry. X-ray structural analysis. Fluorescence analysis of the substances. Microscopy, its types. Potentiometry. Computer identification of compounds.
Assessment methods and criteria: Written-oral control -30 points; Final control (exam) - 70 points
Recommended books: 1. Зінчук В. К., Левицька Г. Д., Дубенська Л. О. Фізико-хімічні методи аналізу. Навч. Посібник. — Л.: В-во ЛНУ ім. І. Франка, 2008. — 361 с. 2. Воловенко Ю. М. Ядерний магнітний резонанс/ Ю. М. Воловенко, О. В. Туров // К.: Перун, 2007. — 476 с. 3. Лебедев А. М. Мас-спектрометрия органических веществ. — М.: 2005, 520 с. 5. Ионин Б. И., Ершов Б. А., Кольцов А. И. ЯМР-спектроскопия в органической химии. — Л.: Химия, Л/О, 1983. — 269 с. 6. Сергеев Н. И. Спектроскопия ЯМР. — М.: Из-во МГУ, 1981. — 279 с. 7. Гюнтер Х. Введение в курс спектроскопии ЯМР. — М. : Мир, 1984. — 478 с. 8. Нифантьев И. Э., П. В. Ивченко. Практический курс спектроскопии ядерного магнитного резонанса. Методическая разработка. Москва, 2006. — 200 с. 9. Магнитный резонанс в медицине /под ред. П. Ринка. — Oxford, Backwell scientific publications, 1995, 247 c. 10. Хорнак Д. Основы МРТ. — 1999. — 233 с. 11. Mitchell T., Costisella B. NMR — from spectra to structure. Springer-Verlag Berlin Heidelberg, 2007. — 220 p.