Home/ Majors directory/Biotechnology and Bioengineering/Biochemistry of Microorganisms and Computer Technology in Industrial Biotechnology
Biochemistry of Microorganisms and Computer Technology in Industrial Biotechnology
Major: Biotechnology and Bioengineering
Code of subject: 6.162.02.E.066
Credits: 7.00
Department: Technology of Biologically Active Substances, Pharmacy and Biotechnology
Lecturer: Associate Professor, Ph.D. Veronika Chervetsova
Ph.D., Associate professor Khomyak S.V.
Semester: 6 семестр
Mode of study: денна
Завдання: The ability to solve complex specialized tasks and practical problems characterized by the complexity and uncertainty of conditions in biotechnology and bioengineering, or in the learning process that involves the application of theories and methods of biotechnology and bioengineering.
general competences:
ЗК04. Skills in using information and communication technologies
professional competences:
ЗK 14. The ability to use modern automated systems for managing the production of biotechnological products for various purposes, their technical, algorithmic, informational and software support for solving professional tasks.
ФК 04. The ability to understand and apply mathematical modeling to study modes, conditions and regularities of microbiological processes.
ФКO 2.4 Ability to apply knowledge of biochemical transformations in the cells of microorganisms, as well as for the development of enzyme preparation technologies.
ФКO 2.10 The ability to demonstrate knowledge of the basics in the field of information technologies for the analysis of biotechnological production systems and the construction of bioobjects and predictive biotesting.
Learning outcomes: know:
- how computer technologies are used for storage, cultivation, identification of microorganisms, for conducting a variety of biochemical, microscopic studies, pharmacogenomics, in the search for new drugs;
- have in-depth knowledge of the equipment used in the biotechnological analytical laboratory;
be able:
- work with biotechnological computer programs, build structural formulas and 3D models of biologically active compounds, name them, calculate molecular weight, proton spectra, dissociation constants
- to study the biological activity of drugs using computer programs
- to analyze microscopic preparations of industrial microorganisms with the help of computer technologies
- search for biotechnological information via the Internet
- apply the acquired knowledge and understanding for the introduction of new technologies;
Required prior and related subjects: Informatics
Mathematical modeling of microbiological processes
General biotechnology
Bioinformatics
Modern application of biotechnological methods
Biomonitoring, biosafety, biosecurity and eco-examination
Summary of the subject: Statistical processing of results. The general population. Presentation of experimental data. The law of distribution. General population and main statistical indicators to characterize the data set.
Basic concepts of analysis of variance. Analysis of variance of the influence of certain factors on the studied indicator. One-way analysis of variance. Two-factor analysis of variance. Statistical method of analysis of ANOVA results.
Programs for molecular modeling and molecular design.
Classification of sensors. Analytical characteristics of sensors. Physico-chemical processes on the sensitive surface of sensors for indication of biologically active compounds and methods of modification of target properties of these sensors.
Analyzers for clinical diagnosis. Portable analyzers for clinical diagnosis at home. Systems for "in vivo" monitoring in clinical settings.
Enzymes biosensors, sensors based on microorganisms. Biosensors based on plant and animal tissues. Trends in the field of sensory integration.
Pharmacoinformatics, purpose, tasks, directions of use. Evidence-based pharmacy. Assessment of the quality of clinical information in accordance with the technologies of evidence-based medicine. Detailed review of medical databases.
The current state and capabilities of software for analysis and modeling in ecology. The use of GIS technologies in environmental activities.
Computer modeling of biomolecules and nanomaterials. Bioinformation aspects of genomics. Development of joint network platforms for nanobiotechnology.
Опис: Nutrition of microorganisms
Chemical composition of the cell of microorganisms. Macro- and microelements. The need of microorganisms in the sources of Carbon, Nitrogen, Phosphorus. Additional growth factors. Autotrophs and heterotrophs. Types of nutrition of microorganisms. Mechanisms of entry of nutrients into the cell. Removal of products of exchange.
Cultivation and growth of microorganisms
Cumulative cultures of microorganisms and the principle of electivity. Pure cultures, their meaning and methods of production. Methods of cultivation of microorganisms: surface, deep, periodic, continuous. Patterns of growth during periodic cultivation. Growth rate and generation time. Continuous cultivation and its importance in modern biotechnology. The concept of immobilization of enzymes and microbial cells. The main types of nutrient media. Methods of sterilization in laboratory and industrial conditions
Exchange of substances (metabolism) in microorganisms
Biosynthetic processes. Energy processes. Methods of obtaining energy by microorganisms. Fermentation, respiration, photosynthesis, chemosynthesis. The main ways of oxidation of carbohydrates: Embden-Meyerhoff-Parnas, Warburg-Dickens-Horeker, Entner-Dudorov.
Aerobic oxidation of various organic substances. Respiration (Krebs cycle and respiratory chain)
Aerobic oxidation of various organic substances. Breathing (Krebs cycle and respiratory chain). The phenomenon of oxidative phosphorylation. Complete and incomplete oxidation. Microbiological transformation of organic compounds. Nitrate, sulfate, carbonate respiration. Representatives and importance of anaerobic respiration in nature.
Regulation of metabolism in microorganisms
Regulation of enzyme activity. Properties of allosteric enzymes. Covalent modification of enzymes.
Regulation of enzyme synthesis by induction and repression. Concept of operon. Catabolite repression, repression by the end product. Synthesis of enzymes of central metabolic pathways.
Development of information and computer technologies in biotechnology. Information technologies in NBIC - convergence of sciences. Application of programs in production processes.
Computer programs for building structural formulas and working with spectra. Work with programs ACD Lab, MestReNova, Nuts, Isis Draw. Construction of structural formulas and 3D models of biologically active compounds, their names. Computer analysis of predicted spectra of nuclear magnetic resonance, infrared and ultraviolet spectra, mass spectrometry.
Computer technologies in the control of production parameters. Control of the process, consumption of raw materials and production parameters in the production of flour, bakery products, macaroni, confectionery products.
Computer technologies in alcohol production. Control of the process, costs and production parameters in the production of alcohol, wine, beer and in other fermentation technologies.
Computer technologies in dairy production. Control of the process of consumption of raw materials and production parameters in the production of milk, butter, cheese, ice cream, and sour milk products.
Stages of designing technological schemes.
Computer programs for obtaining and processing infrared spectra Spectrum and Spectrum Quant.
Assessment methods and criteria: Execution and defense of laboratory works - 20 points;
Calculation and graphic work - 30 points.
Test - 50 points.
Критерії оцінювання результатів навчання: The main methods of knowledge diagnosis are: current (PC) and semester control (SC), which is carried out from educational material, the scope of which is determined by the work program of the discipline for the semester.
Current control is carried out during lectures and practical classes in order to check the level of assimilation of theoretical and practical knowledge and skills of the student. PC is conducted in the form of: written and oral control and defense of practical works.
Semester control is conducted in the form of an exam. The exam is a form of SC of the student's training results exclusively for all types of work provided by the program of the academic discipline.
Порядок та критерії виставляння балів та оцінок: 100-88 points - certified with an “excellent” grade - High level: the student demonstrates an in-depth mastery of the conceptual and categorical apparatus of the discipline, systematic knowledge, skills and abilities of their practical application. The mastered knowledge, skills and abilities provide the ability to independently formulate goals and organize learning activities, search and find solutions in non-standard, atypical educational and professional situations. The applicant demonstrates the ability to make generalizations based on critical analysis of factual material, ideas, theories and concepts, to formulate conclusions based on them. His/her activity is based on interest and motivation for self-development, continuous professional development, independent research activities, implemented with the support and guidance of the teacher. 87-71 points - certified with a grade of “good” - Sufficient level: involves mastery of the conceptual and categorical apparatus of the discipline at an advanced level, conscious use of knowledge, skills and abilities to reveal the essence of the issue. Possession of a partially structured set of knowledge provides the ability to apply it in familiar educational and professional situations. Aware of the specifics of tasks and learning situations, the student demonstrates the ability to search for and choose their solution according to the given sample, to argue for the use of a particular method of solving the problem. Their activities are based on interest and motivation for self-development and continuous professional development. 70-50 points - certified with a grade of “satisfactory” - Satisfactory level: outlines the mastery of the conceptual and categorical apparatus of the discipline at the average level, partial awareness of educational and professional tasks, problems and situations, knowledge of ways to solve typical problems and tasks. The applicant demonstrates an average level of skills and abilities to apply knowledge in practice, and solving problems requires assistance, support from a model. The basis of learning activities is situational and heuristic, dominated by motives of duty, unconscious use of opportunities for self-development. 49-00 points - certified with a grade of “unsatisfactory” - Unsatisfactory level: indicates an elementary mastery of the conceptual and categorical apparatus of the discipline, a general understanding of the content of the educational material, partial use of knowledge, skills and abilities. The basis of learning activities is situational and pragmatic interest.
Recommended books: 1. M.E. Solovyov, M.M. Solovyov. Computer chemistry. // "Solon-press". -2005 - 536p.
2. Orlov V.D., Lipson V.V., Ivanov V.V. Medical chemistry // Folio.- 2005.- 464 S.
3. Sean Ekins. Computer applications in pharmaceutical research and development // Wiley.- 2016.- 806 p.
4. White IA, Kleschev M.F. Biological and chemical sensor systems // Kharkiv NTU "KhPI". - 2011. - 144 p.
5. Lakhno V.D. (ed) -Computers and supercomputers in biology // Mosuva 2002. - 560 p.
6. Bultinck P., De Winter H., Langenaeker W., Tollenaere J.P. Computational Medicinal Chemistry for Drug Discovery // New York.- Marcel Dekker Inc.- 2014.- 684 p.
7. Abraham D.J. Burgers Medicinal Chemistry and Drug Discovery. Vol. 1. Drug Discovery. // Wiley.- 2013.- 932 P