Physical Methods of Analysis of Drugs

Major: Pharmacy, Industrial Pharmacy
Code of subject: 6.226.01.E.089
Credits: 4.00
Department: Technology of Biologically Active Substances, Pharmacy and Biotechnology
Lecturer: Assistant, Ph.D., Senior researcher Semen Khomyak
Semester: 8 семестр
Mode of study: денна
Learning outcomes: Knowledge of the chemical structure of medicinal substances, sources and methods of their obtaining, regularities of the relationship between chemical structure and pharmacological properties, methods of identification and quantitative recognition, storage conditions. To be able to use the methods of quality control of raw materials and finished pharmaceutical products and to conduct qualitative and quantitative analysis of substances of inorganic, organic and biological origin. To be able to generalize and systematize information about the chemical structure, physical properties, methods of obtaining and analyzing modern medicinal substances.
Required prior and related subjects: Prerequisites: Physical methods of analysis of substances. Methods of organic synthesis. Pharmaceutical chemistry Co-requisites: Pharmacy Technology of Medicines Fundamentals of emergency medical care
Summary of the subject: Concept and definition of instrumental methods of analysis of drugs. Methods of determining the melting point. Distillation. Determination of relative density. Potentiometric and colorimetric method for determining pH. Potentiometric titration. Polarography. Refractometry. Refractive index. Polarimetry. Measuring optical rotation. Photometric methods. Law of Bouguer-Lambert-Ber. Spectrophotometry. Absorption spectrum. UV-visible and IR spectrophotometry. Optical density. Methods of determining the concentration of solutions using spectrophotometry. Basic schemes of photocolorimeters. Nuclear magnetic resonance. Instruments for NMR registration. Resonance frequency. Chemical shift. Spin-spin coupling. Interpretation of NMR spectra. Spectrum processing programs. X-ray Spectroscopy. Mass Spectrometry. Chromatography. Types of chromatography. Adsorbents and eluents for chromatography. Liquid and gas chromatography. Thin layer chromatography and paper chromatography. Ion exchange, affinity, adsorption chromatography.
Assessment methods and criteria: • written report from laboratory work, oral questioning (10 points), current tests (30 points) • final control (exam) - 60 points (written-oral form).
Recommended books: 1. Yu.M. Volovenko Nuclear magnetic resonance / Yu.M. Volovenko, O.V. Turov // K .: Perun, 2007. 476p. 2. D.О. Melnychuk, S.D. Melnychuk, V.M. Voytsitskiy, V.А. Grytsenko, L.G. Kalachnuk, S.V. Khyznyak, Analitychny methody doslidzen, spektroskopichni: teorethychny osnovy and methodyky: К. – 2016. – 289с. 3. E.Prech, F. Bullmann, K.Affolter. Determination of the structure of organic compounds, M., Mir, 2012, - 438p. 4. Zinchuk V.K., Levytska G.D., Dubenska L.O. Physico-chemical methods of analysis: A manual. Lviv: Publishing Center of Ivan Franko National University, 2008. 362 p. 5. Elementary organic spectroscopy, principles and chemical applications.- Y.R. Sharma. - New Delhi: S. CHAND & COMPANY PVT. LTD, 2013. – 385р. 6. Pharmatsevtychna himiya/ P.О. Bezugliy, V.А. Georgiyants, І.С. Grycenko. – Vinnytsa: Nova knyga, 2017.- 456p. 5. Elementary organic spectroscopy, principles and chemical applications.- Y.R. Sharma. - New Delhi: S. CHAND & COMPANY PVT. LTD, 2013. – 385р. 6. Pharmatsevtychna himiya/ P.О. Bezugliy, V.А. Georgiyants, І.С. Grycenko. – Vinnytsa: Nova knyga, 2017.- 456p.