Biotechnology of Enzyme Diagnostics

Major: Biotechnology and Bioengineering
Code of subject: 7.162.03.E.028
Credits: 4.00
Department: Technology of Biologically Active Substances, Pharmacy and Biotechnology
Lecturer: Havryliak Viktoria, D. Sc., Prof. Stadnytska Natalia, PhD, Ass. prof.
Semester: 2 семестр
Mode of study: денна
Learning outcomes: • Ability to plan and perform experimental work in the field of biotechnology using modern equipment and methods, to interpret the data on the basis of a set of modern knowledge and ideas about the object and subject of research, to draw sound conclusions .; • Ability to use about enzymes and enzyme preparations, understanding of technologies for designing bioorganic catalysts with specified properties based on enzymes or their complexes. • Have the skills of isolation, identification, storage, cultivation, immobilization of biological agents, optimize nutrient media, choose the best methods of analysis, isolation and purification of the target product, using modern biotechnological methods and techniques specific to a particular area of ??biotechnology. • Knowledge of molecular processes in enzymology for the design of biological agents and the development of new and improvement of existing applied biotechnologies of enzyme preparations in various fields.
Required prior and related subjects: prerequisites: Modern application of biotechnological methods (bioindustry) co-requisites: Biotechnology of immunopreparations and probiotics Biotechnology of agricultural and veterinary drugs
Summary of the subject: The concept of enzyme diagnosticum. Characteristics of technology of production of immunobiological drugs. Basic principles of development diagnostic kits for the identification of microorganisms. Biosensors, their types. Basic principles of designing biosensors.
Assessment methods and criteria: • oral questioning, written reports on laboratory works – 20 points; • final control (exam) – 80 points (written-oral form).
Recommended books: Біохімія ензимів / М.М. Марченко, Л.В. Худа, М.М. Великий, Л.І. Остапченко. – Чернівці: Чернівецький національний університет, 2012. – 416 с. Ензимологія / Укл. Худа Л.В. - Чернівці: Чернівецький національний університет, 2009. – 80 с. Галкін Б.М., Іваниця В.О., Галкін М.Б. Інженерна ензимологія. – Одеса: ОНУ.- 2017. – 103 с. Practical Enzymology /Bisswanger H. - 2011 Wiley-VCH Verlag & Co. KGaA