Biochemistry of Microorganisms and Computer Technology in Industrial Biotechnology

Major: Biotechnology and Bioengineering
Code of subject: 6.162.02.E.066
Credits: 7.00
Department: Technology of Biologically Active Substances, Pharmacy and Biotechnology
Lecturer: Associate Professor, Ph.D. Veronika Chervetsova Ph.D., Associate professor Khomyak S.V.
Semester: 6 семестр
Mode of study: денна
Мета вивчення дисципліни: The purpose of teaching the course is to prepare a technological engineer of the biotechnological industry for the active use of knowledge and skills in the methods of de novo isolation of new producers, the study of biochemical features of isolated microorganisms, in particular bacteria and mold fungi, as well as their application in biotechnological processes. As a result of studying the discipline, the student will gain knowledge about the application of computer programs in the production process, in particular biotechnological; as well as directions for the application of programs for obtaining technological indicators, in particular when controlling production parameters.
Завдання: The ability to solve complex specialized tasks and practical problems characterized by the complexity and uncertainty of conditions in biotechnology and bioengineering, or in the learning process that involves the application of theories and methods of biotechnology and bioengineering. general competences: ЗК04. Skills in using information and communication technologies professional competences: ЗK 14. The ability to use modern automated systems for managing the production of biotechnological products for various purposes, their technical, algorithmic, informational and software support for solving professional tasks. ФК 04. The ability to understand and apply mathematical modeling to study modes, conditions and regularities of microbiological processes. ФКO 2.4 Ability to apply knowledge of biochemical transformations in the cells of microorganisms, as well as for the development of enzyme preparation technologies. ФКO 2.10 The ability to demonstrate knowledge of the basics in the field of information technologies for the analysis of biotechnological production systems and the construction of bioobjects and predictive biotesting.
Learning outcomes: know: - how computer technologies are used for storage, cultivation, identification of microorganisms, for conducting a variety of biochemical, microscopic studies, pharmacogenomics, in the search for new drugs; - have in-depth knowledge of the equipment used in the biotechnological analytical laboratory; be able: - work with biotechnological computer programs, build structural formulas and 3D models of biologically active compounds, name them, calculate molecular weight, proton spectra, dissociation constants - to study the biological activity of drugs using computer programs - to analyze microscopic preparations of industrial microorganisms with the help of computer technologies - search for biotechnological information via the Internet - apply the acquired knowledge and understanding for the introduction of new technologies;
Required prior and related subjects: Informatics Mathematical modeling of microbiological processes General biotechnology Bioinformatics Modern application of biotechnological methods Biomonitoring, biosafety, biosecurity and eco-examination
Summary of the subject: Statistical processing of results. The general population. Presentation of experimental data. The law of distribution. General population and main statistical indicators to characterize the data set. Basic concepts of analysis of variance. Analysis of variance of the influence of certain factors on the studied indicator. One-way analysis of variance. Two-factor analysis of variance. Statistical method of analysis of ANOVA results. Programs for molecular modeling and molecular design. Classification of sensors. Analytical characteristics of sensors. Physico-chemical processes on the sensitive surface of sensors for indication of biologically active compounds and methods of modification of target properties of these sensors. Analyzers for clinical diagnosis. Portable analyzers for clinical diagnosis at home. Systems for "in vivo" monitoring in clinical settings. Enzymes biosensors, sensors based on microorganisms. Biosensors based on plant and animal tissues. Trends in the field of sensory integration. Pharmacoinformatics, purpose, tasks, directions of use. Evidence-based pharmacy. Assessment of the quality of clinical information in accordance with the technologies of evidence-based medicine. Detailed review of medical databases. The current state and capabilities of software for analysis and modeling in ecology. The use of GIS technologies in environmental activities. Computer modeling of biomolecules and nanomaterials. Bioinformation aspects of genomics. Development of joint network platforms for nanobiotechnology.
Опис: Nutrition of microorganisms Chemical composition of the cell of microorganisms. Macro- and microelements. The need of microorganisms in the sources of Carbon, Nitrogen, Phosphorus. Additional growth factors. Autotrophs and heterotrophs. Types of nutrition of microorganisms. Mechanisms of entry of nutrients into the cell. Removal of products of exchange. Cultivation and growth of microorganisms Cumulative cultures of microorganisms and the principle of electivity. Pure cultures, their meaning and methods of production. Methods of cultivation of microorganisms: surface, deep, periodic, continuous. Patterns of growth during periodic cultivation. Growth rate and generation time. Continuous cultivation and its importance in modern biotechnology. The concept of immobilization of enzymes and microbial cells. The main types of nutrient media. Methods of sterilization in laboratory and industrial conditions Exchange of substances (metabolism) in microorganisms Biosynthetic processes. Energy processes. Methods of obtaining energy by microorganisms. Fermentation, respiration, photosynthesis, chemosynthesis. The main ways of oxidation of carbohydrates: Embden-Meyerhoff-Parnas, Warburg-Dickens-Horeker, Entner-Dudorov. Aerobic oxidation of various organic substances. Respiration (Krebs cycle and respiratory chain) Aerobic oxidation of various organic substances. Breathing (Krebs cycle and respiratory chain). The phenomenon of oxidative phosphorylation. Complete and incomplete oxidation. Microbiological transformation of organic compounds. Nitrate, sulfate, carbonate respiration. Representatives and importance of anaerobic respiration in nature. Regulation of metabolism in microorganisms Regulation of enzyme activity. Properties of allosteric enzymes. Covalent modification of enzymes. Regulation of enzyme synthesis by induction and repression. Concept of operon. Catabolite repression, repression by the end product. Synthesis of enzymes of central metabolic pathways. Development of information and computer technologies in biotechnology. Information technologies in NBIC - convergence of sciences. Application of programs in production processes. Computer programs for building structural formulas and working with spectra. Work with programs ACD Lab, MestReNova, Nuts, Isis Draw. Construction of structural formulas and 3D models of biologically active compounds, their names. Computer analysis of predicted spectra of nuclear magnetic resonance, infrared and ultraviolet spectra, mass spectrometry. Computer technologies in the control of production parameters. Control of the process, consumption of raw materials and production parameters in the production of flour, bakery products, macaroni, confectionery products. Computer technologies in alcohol production. Control of the process, costs and production parameters in the production of alcohol, wine, beer and in other fermentation technologies. Computer technologies in dairy production. Control of the process of consumption of raw materials and production parameters in the production of milk, butter, cheese, ice cream, and sour milk products. Stages of designing technological schemes. Computer programs for obtaining and processing infrared spectra Spectrum and Spectrum Quant.
Assessment methods and criteria: Execution and defense of laboratory works - 20 points; Calculation and graphic work - 30 points. Test - 50 points.
Критерії оцінювання результатів навчання: The main methods of knowledge diagnosis are: current (PC) and semester control (SC), which is carried out from educational material, the scope of which is determined by the work program of the discipline for the semester. Current control is carried out during lectures and practical classes in order to check the level of assimilation of theoretical and practical knowledge and skills of the student. PC is conducted in the form of: written and oral control and defense of practical works. Semester control is conducted in the form of an exam. The exam is a form of SC of the student's training results exclusively for all types of work provided by the program of the academic discipline.
Recommended books: 1. M.E. Solovyov, M.M. Solovyov. Computer chemistry. // "Solon-press". -2005 - 536p. 2. Orlov V.D., Lipson V.V., Ivanov V.V. Medical chemistry // Folio.- 2005.- 464 S. 3. Sean Ekins. Computer applications in pharmaceutical research and development // Wiley.- 2016.- 806 p. 4. White IA, Kleschev M.F. Biological and chemical sensor systems // Kharkiv NTU "KhPI". - 2011. - 144 p. 5. Lakhno V.D. (ed) -Computers and supercomputers in biology // Mosuva 2002. - 560 p. 6. Bultinck P., De Winter H., Langenaeker W., Tollenaere J.P. Computational Medicinal Chemistry for Drug Discovery // New York.- Marcel Dekker Inc.- 2014.- 684 p. 7. Abraham D.J. Burgers Medicinal Chemistry and Drug Discovery. Vol. 1. Drug Discovery. // Wiley.- 2013.- 932 P